BIOL 1406: LAB EXERCISE 3

Objectives

A.  Understanding pH and buffers

B.  Introduction to the brightfield microscope

After completing this exercise, you should be able to:

• Define and correctly use the following terms: dissociation, acid, base, pH, buffer, buffering range, and buffering capacity.

• Explain how solutions with different pH compare with respect to their concentrations of H⁺ and OH^-

• Calibrate and use a pH meter to measure and adjust the pH of a solution.

• Prepare buffered solutions and determine the pH range at which a solution acts as a buffer.

• Plot and interpret a pH titration curve for a solution.

• Identify the various parts of the brightfield microscope and describe the correct way to use and care for it. 

• Describe the particular features of the brightfield microscopes available in your lab.

• Determine the power of magnification when viewing objects with the brightfield microscope.

• View both wet mounts and prepared slides using the brightfield microscope.