BIOL 1406: LAB EXERCISE 7

Objectives

After completing this exercise, you should be able to:

• Explain how chromatography is used to separate a mixture of molecules into its components, and describe the role of the mobile and stationary phases in this process.

• Explain how thin layer chromatography works, and be able to use it to separate a mixture of molecules.

• Explain what a scanning spectrophotometer is used for, and discuss how the information it provides is useful to scientists.

• Plot and interpret an absorption spectrum using a scanning spectrophotometer.

• Explain how absorption spectra can be used to identify molecules.