BIOL 1406: LAB EXERCISE 8b

Objectives

After completing this exercise, you should be able to:

• Explain what a protein assay is, and discuss why it is an important part of the purification process.

• Distinguish between a qualitative assay and a quantitative assay.

• Discuss the advantages and disadvantages of the following assays for protein concentration: A280 measurements, the biuret assay, the Lowry assay, and the Bradford assay.

• Perform a Bradford assay and make a standard curve to determine the concentration of protein in each of your milk fractions.