BIOL 1406: LAB EXERCISE 9a

Objectives

After completing this exercise, you should be able to:

• Define gene cloning, recombinant DNA technology, and genetic engineering.

• Describe how genes are cloned, and explain the usefulness of gene cloning.

• Explain the role of vectors, restriction enzymes, and DNA ligase in genetic engineering.

• Give examples of different types of vectors used in genetic engineering.

• Describe the process of transformation and carry out this procedure in the laboratory.

• Inoculate agar plates with bacteria.

• Describe the function of a selectable marker in the isolation of transformants.

• Perform a restriction digest of plasmids.

• Pour and run an agarose gel to separate DNA fragments produced by a restriction digest.