BIOL 1406: LAB EXERCISE 9b

Objectives

After completing this exercise, you should be able to:

• Analyze an agarose gel to determine the number of different-sized DNA fragments present in a sample.

• Analyze an agarose gel to determine the approximate size of each DNA fragment

• Determine which agar plates were inoculated with transformed bacteria and which were inoculated with bacteria that were not transformed.

• Examine colonies of transformed E. coli, to identify the colonies that carry a non-recombinant plasmid and the colonies that carry a recombinant plasmid.