BIOL 1406
PreLab 6.2
How can I prepare solutions with different substrate
concentrations?
The first enzyme you will study in lab is glucose oxidase. One of the substrates
for glucose oxidase is glucose. In order to test how substrate concentration
affects glucose oxidase activity, you will need to prepare 6 solutions
containing different concentrations of glucose.
| Your Turn | ||
| Starting with a stock solution containing 60 mg/dL of glucose, and using dH2O as your diluting solution, you need to make 3 mL of each of the following glucose solutions: 30 mg/dL, 15 mg/dL, 7.5 mg/dL, 3.75 mg/dL, 1.875 mg/dL, and 937.5 µg/dL. Fill in the information below to show how you would prepare these solutions using a serial dilution. If you forgot how to do the calculations for a serial dilution, please consult Prelab 2.6. | ||
| What is df for this dilution series? | Hint | Check your answer. |
| What is V2 for this dilution series? | Hint | Check your answer. |
| What is V1 for this dilution series? | Hint | Check your answer. |
| Now, in the boxes provided below the following diagram, fill in the information needed to show how you would make the required solutions. For each vertical arrow (A and B), enter the volume and type of liquid you will place in each tube (e.g. 13 mL of stock solution or 9 mL of dH2O.) For each horizontal arrow (C through I), enter how much solution you will transfer from one tube to the next (e.g. 5 mL). You will need this information during lab, so record it in your lab notebook as well. | ||
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| Arrow A = | Hint | Check your answer. |
| Arrow B = | Hint | Check your answer. |
| Arrow C = | Hint | Check your answer. |
| Arrow D = | Hint | Check your answer. |
| Arrow E = | Hint | Check your answer. |
| Arrow F = | Hint | Check your answer. |
| Arrow G = | Hint | Check your answer. |
| Arrow H = | Hint | Check your answer. |
| Arrow I = | Hint | Check your answer. |
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